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	Provedor de dados ArchiMer (1) Autor Goux, Didier (1) Ledauphin, Jerome (1) Orvain, Francis (1) Takahashi, Eri (1) Palavra-chave Biofilm (1) Cell lysis (1) Dowex (1) GCMS analysis (1) Immuno staining (1) Immunostaining (1) Intertidal sediments (1) Navicula jeffreyi (1) Protein (1) Transmission electron microscopy (1) Mais... Tipo do documento Text (1) Ano 2009 (1) País France (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Optimising extraction of extracellular polymeric substances (EPS) from benthic diatoms: comparison of the efficiency of six EPS extraction methods ArchiMer Takahashi, Eri; Ledauphin, Jerome; Goux, Didier; Orvain, Francis. There is no universal method that can be applied to extract bound extracellular polymeric substances (EPS) from benthic diatoms of intertidal sediments without causing cell lysis. Six extraction methods were tested on a diatom culture of Navicula jeffreyi to establish the best compromise between high yields of carbohydrate extraction and minimum cell lysis. Extraction with distilled water provoked cell lysis (as already known). The five other extraction methods (dowex resin, artificial seawater of half salinity and extractions after pretreatment with gluteraldehyde by three methods: water, dowex water and dowex buffer) did not provoke cell lysis as shown by transmission electronic microscopy. This result was confirmed by the minimum release of internal... Tipo: Text Palavras-chave: Transmission electron microscopy; Protein; Navicula jeffreyi; Intertidal sediments; Immuno staining; Immunostaining; GCMS analysis; Dowex; Cell lysis; Biofilm. Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7418.pdf Registros recuperados: 1 Primeira ... 1 ... Última

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